Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Primers and probes used for quantitative methylation-specific polymerase chain reaction (qMSP)

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Polymerase Chain Reaction, Sequencing

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: MGMT methylation frequencies and methylation level in methylated glioma samples

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, CpG Methylation Assay

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Overall survival in primary glioblastoma patients treated with standard radiotherapy and concomitant temozolomide . ( A ) Methylation status based on results from qMSP. Blue line; methylated, red line; unmethylated. ( B ) Methylation status based on results from pyrosequencing. Blue line; methylated, red line; unmethylated. ( C ) Methylation status based on both qMSP and pyrosequencing results. Blue line; methylated in both methods, red line; unmethylated in both methods, green line; methylated in pyrosequencing assay and unmethylated in qMSP assay. Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Pyrosequencing Assay

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Patient characteristics and results of survival analysis for primary glioblastoma patients when stratified by MGMT methylation status

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Methylation, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Gene expression level of MGMT is associated with promoter DNA methylation status . MGMT gene expression in methylated (blue box plots) and unmethylated (red box plots) tissue samples analyzed by two different primer/probe sets (Assay 1; Hs00172470_m1 and assay 2; Hs01037698_m1). ( A ) Methylation status based on results from qMSP. ( B ) Methylation status based on results from pyrosequencing. Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Gene Expression, DNA Methylation Assay, Methylation

Journal: Journal of Translational Medicine

Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

doi: 10.1186/1479-5876-10-36

Figure Lengend Snippet: Scatter plots of MGMT gene expression quantity in methylated and unmethylated samples . Red color indicates samples with negative methylation status by qMSP and pyrosequencing, blue color indicates samples with positive methylation status by qMSP and pyrosequencing, and green color indicate samples with non-concordant methylation status in the qMSP and pyrosequencing analysis. Circles indicate samples with loss of 10q26, whereas dots represent samples without loss of this region. Plots are based on the normalized gene expression detected by primer/probe set Hs00172470_m1. The plots based on the normalized gene expression detected by the other primer/probe set, Hs01037698_m1, were similar (data not shown). Abbreviation: qMSP, quantitative methylation specific PCR

Article Snippet: However, the survival curve differences are more distinct when using the pyrosequencing based methylation status, implying that this is the better method to use for estimating the prognosis.

Techniques: Gene Expression, Methylation

Journal: Neuro-Oncology Advances

Article Title: Clinical value of the MGMT promoter methylation score in IDHmt low-grade glioma for predicting benefit from temozolomide treatment

doi: 10.1093/noajnl/vdae224

Figure Lengend Snippet: Association between progression-free survival (PFS) and the MGMTp methylation score. The association of the MGMTp methylation score, based on the MGMT-STP27 procedure, with progression-free survival (PFS; interval from diagnosis) was evaluated by the hazard ratio (confidence interval at 95%) from the cox regression model. The forest plots in A and B correspond to the PFS reported for patients treated in EORTC-22033 with temozolomide (TMZ) or radiotherapy (RT), respectively. , The MGMT-STP27 score was significant in the TMZ treated patients, but not in the patients with RT. The forest plots in C and D visualize the outcome of the Montpellier patients treated with TMZ, with time to progression defined by the next treatment-free survival (NxtTrtFS), and PFS defined by RANO, respectively. The MGMT-STP27 score was significant in the Montpellier cohort when using NxtTrtFS as outcome measure, a trend was observed when using RANO criteria for PFS. * P <.05; ** P <.01.

Article Snippet: The association between the MGMT-STP27 score and NxtTrtFS was confirmed using a pyrosequencing-based MGMTp methylation score (MGMT-PYROscore) obtained for the same samples ( P = .002, ).

Techniques: Methylation, Biomarker Discovery